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Das, Arunava
- Evaluation of Engineering Properties of Problematic Soils using Bacillus pasteurii
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Authors
Affiliations
1 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, IN
2 Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, IN
3 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, IN
4 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, IN
1 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, IN
2 Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, IN
3 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, IN
4 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2098-2102Abstract
Soil improvement method using enzymatic activity from bacteria Bacillus pasteurii that able to catalyse urea and calcium chloride enabling cementation solution has become an emerging approach to increase the shear strength of sand. The production of urease enzyme of B. pasteurii will be increased using specific medium. Determination of the volume of bacteria needed is based on the soil porosity is studied and analysed. The preliminary result of B. pasteurii that use specific medium could increase the urease enzyme activity and already verified using urease measurement kit. The treatment period required for the initiation of production of cementing material (calcite) has been reduced from one month to 3 days, while using this specific medium for enzyme urease production. This leads to the rapid filling of the soil pores that result in the hardening of soil. The bonding between the soil particles also increases that leads to increase in the density of soil. The final results of shear improvement are obtained by conducting Direct shear test and Unconfined compression test on sandy and silty soils respectively. Finally the cohesion between the soil particles was identified through the permeability test.Keywords
B. pasteurii, Direct Shear Test, Permeability, Soil Pores, Unconfined Compression Test.References
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- In Vitro Antibacterial and Anticancer Study of Bioactive Compounds Isolated from Punica Grantum Peel
Abstract Views :434 |
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Authors
Affiliations
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
Source
Indian Journal of Public Health Research & Development, Vol 11, No 1 (2020), Pagination: 45-52Abstract
Plants have been used as natural medicines since the dawn of human kind. This research was focused on phytochemical analysis of Punica grantum peel extract consist of ethanol: chloroform (99:1) as solvent and its constituents were molecularly characterized by GCMS analysis and its ability as an antioxidant, anticancer against K562 cell and antibacterial against some food borne pathogens which comprises of 5 gram-positive and 3-gram-negative bacteria. The phytochemical analysis was performed biochemically and results reveals the presence of secondary metabolites such as Flavonoids, Alkaloids, Tannins, Saponins, etc. and absence of glycosides. The extract was tested against both gram-positive and gram-negative bacteria by agar well diffusion method and its results have shown that the streptococcus faecalis have shown highest susceptibility against the extract. Its antioxidant potential was quantitatively determined by DPPH assay and results showed high range of antioxidant present in the extract with IC50 as 504.9μg/ml. The cytotoxicity assay was performed with peel extract of different concentration against K562 cancer lines which is a myelogenous leukemia cell line. However, the cytotoxicity is less when compared with cell viability and its effects were mild against the cancer cells.Keywords
Punicagranatum, DPPH, GCMS, K562 Cancer Cells (Myelogenous Leukemia).- In Vitro Anticancer Study of Bioactive Compound Isolated from Musa Extract (Musa acuminata)
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Authors
Affiliations
1 Associate Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
2 Research Associate cum Assistant Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
3 III Year Biotech Students, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
1 Associate Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
2 Research Associate cum Assistant Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
3 III Year Biotech Students, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN